LAUSR

Exposure of ultraviolet B (UVB) radiation is the main factor from the environment to cause skin photoaging. Lactobacillus rhamnosus ATCC 7469, is a probiotic strain with a good track record for enhancing human health. The present study conducted the impacts of heat-killed Lactobacillus rhamnosus ATCC 7469 (RL) on photoaging in vitro using mouse skin fibroblast (MSF) cells and human epidermal melanocytes (HEM) exposed to UVB. The results showed that (1) RL protected UVB-induced cytotoxicity relating to absorb UVB and reduce DNA damage. (2) RL exerted the anti-wrinkle impact involved in two aspects. Firstly, RL down-regulated MMP-1, 2, 3 expressions associating with MAPK signaling, resulting in the increased the protein expression of COL1A1, further booting type I collagen abundant thereby promoting the anti-wrinkle impact in MSF cells. Secondly, RL reduced ROS content, further decreasing oxidative damage relating to Nrf2/Sirt3/SOD2 signaling, thereby promoting the anti-wrinkle impact in MSF cells. (3) RL suppressed tyrosinase and TYRP-2 activity and/or levels associating with PKA/CREB/MITF signaling, thereby promoting anti-melanogenesis impact in HEM cells. In conclusion, our findings suggest that RL could reduce photoaging caused by UVB via anti-wrinkle and anti-melanogenesis properties and may be a potential anti-photoaging beneficial component, which is applied in the cosmetic industry.