LAUSR

The robust adventitious root (AR) system of Nitraria tangutorum facilitates its adaptation to drought stress; however, the molecular mechanisms governing AR formation during drought remain unclear. In this study, we demonstrated that a sucrose (40 g L−1) treatment of N. tangutorum explants for 48 h significantly increased the rate of AR development from 36% in the untreated control group to 100%. Transcriptome analysis revealed sucrose exerts a significant influence on H2O2 homeostasis and hormone signal transduction pathways. Sucrose treatment promoted IAA biosynthesis through the upregulation of TSA, TSB, and YUC genes and suppressed gibberellin (GA) and cytokinin biosynthesis by downregulating CPS, GA3ox, GA20ox, and LOG. Moreover, sucrose induced abscisic acid (ABA) and salicylic acid (SA) accumulation while reducing JA‐Ile, and activated H2O2 signaling through the upregulation of RBOH. Exogenous H2O2 (1–8 mM) mimicked the effects of sucrose; however, this effect was abolished by the auxin transport inhibitor TIBA, indicating that the effects of H2O2 depend on auxin signaling. These results reflect that sucrose acts as an energy substrate as well as a signaling molecule to coordinate hormonal interactions and redox signaling during drought‐adaptive AR formation.