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OR AL PRESENTATIONS/KUR Z VORTR ÄGE 1 | Development of a milkbased lateral flow test for pregnancy diagnosis Entwicklung eines milchbasierten LateralFlowTests zur Trächtigkeitsdiagnose beim Rind T Krebs1; I Wiedemann1; C Blaschka1; C Lenz2,3; M Hennies4; S Kleinhans5; K Mütze5; K Knipper6; C Knorr1; J Tetens1 1Department of Animal Sciences, GeorgAugust University Göttingen, Göttingen, Germany; 2Core Facility Proteomics, Institute of Clinical Chemistry, University Medical Center Göttingen, Göttingen, Germany; 3Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany; 4TECO development GmbH, Rheinbach, Germany; 5Hessischer Verband für Leistungsund Qualitätsprüfung in der Tierzucht e.V., Alsfeld, Germany; 6Fassisi, Gesellschaft für Veterinärdiagnostik und Umweltanalysen mbH, Göttingen, Germany Pregnancyassociated glycoproteins (PAGs) are commonly used molecular markers for early pregnancy in cattle. PAG concentrations in serum and milk rise steadily throughout pregnancy and enable pregnancy diagnosis by day 28 of gestation (Wallace, Pohler, Smith, & Green, 2015). Available PAG detection systems in blood and milk are laboratory based, with the exception of one lateral flow blood test (Fassisi AT GmbH, 2017), which enables pregnancy diagnosis from blood drop samples within minutes directly in the stable. The aim of the present study is to develop a similar lateral flow assay for PAG in milk. To this end, cotyledon tissue samples were collected from an abattoir. Samples clustered into four different pregnancy stages: 1. day 20–90 (n = 46), 2. day 91–180 (n = 59), 3. day 181–240 (n = 18) and 4. day 240calving (n = 5). Gestation stages were estimated by measuring the crownrumplength of the fetuses. PAGs were purified from all gestation stages by FPLC. Identification of PAGs in FPLC fractions was achieved by mass spectrometry. Antisera were raised against purified PAG fractions for each gestation stage. Ten different PAGs were indentified in the third gestation stage. The ability to detect PAGs in blood and milk will be tested in samples from cows throughout pregnancy that were collected on eleven different farms. The first results (obtained from 44 samples) indicate a good detection capacity of the antisera in a sandwichELISA (LOD = 15 pg/mL in serum) and a high correlation (R2 = 0.91) of measured results with an established test system. 2 | Effect of glyphosate on bovine oocyte development during in vitro maturation Einfluss des Wirkstoffes Glyphosat auf die Entwicklungskompetenz boviner Oozyten während der In-vitroMaturation S Diers1; C Blaschka1; A Silbersdorff2; C Knorr1; J Tetens1 1Department of Animal Sciences, Georg-August-University Göttingen, Göttingen, Germany; 2Department of Economics, GeorgAugustUniversity Göttingen, Göttingen, Germany Glyphosate is the most widely used agent for nonselective herbicides worldwide (Benbrook 2016, Environ Sci Eur, 28:3). The aim of this study is to determine, whether the active ingredient alone or in formulation with Roundup, the global market leader of glyphosatebased herbicides, affects the maturation and further development of bovine oocytes. Ovaries of healthy cows were collected at a slaughterhouse. The selected cumulusoocytecomplexes matured for 24 hours. Glyphosate (96%) and Roundup were added to the maturation medium in concentrations of 1 μg/mL and 10 μg/mL each. After 24 hours of incubation (39°C, 5% CO2), the oocytes were stained with Hoechst 33 342 to detect the metaphase II stage. The maturation rates of the three tested groups Glyphosate, Roundup and Control showed no significant differences irrespective of the used concentrations. On day seven and eight postfertilization the embryos were evaluated morphologically. The development rates also showed no significant differences between test groups and concentrations. After addition of 1 μg/mL, the average rates of development on day eight were 19.2% (± 10,8%) for pure Glyphosate (n = 301), 22.6% (± 12.3%) for Roundup (n = 315) and 24.1% (± 9.4%) for Control (n = 295). After adding 10 μg/mL to the maturation medium, the developmental averages on day eight were 26.9% (± 14.7%) for Glyphosate (n = 523), 24.1% (± 12.6%) for Roundup (n = 479) and 25.6% (± 11.0%) for Control (n = 389). In this study, negative effects of glyphosate and Roundup on bovine oocyte development could not be detected so far, further analysis will follow. DOI: 10.1111/rda.13387