LAUSR

The aim of this study was to investigate the presence of Leishmania sp. DNA and anti-Leishmania spp. antibodies in free-ranging Sapajus nigritus from an urban forest located in a city in the North Central region of the state of Paraná. For the indirect diagnosis, the Direct Agglutination Test (DAT) was used with promastigote forms of Leishmania (V.) braziliensis, where it was possible to detect the agglutination reaction in 53.33% of the Sapajus nigritus blood samples. For direct diagnosis, the samples were submitted to Real-Time Quantitative Polymerase Chain Reaction (qPCR) which confirmed the presence of Leishmania spp DNA in 26.66% of the tested samples. It reinforces the importance of considering the concept of One Health in the face of diseases with high prevalence, such as leishmaniasis and the need for health education measures. This result show that the animals in the present study have a role as environmental bioindicators for leishmaniasis. This article is protected by copyright. All rights reserved.