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Massive autoimmune hemolysis documented by monocyte monolayer assay in a multiply transfused patient using reticulocytes isolated by simple centrifugation in microhematocrit tubes

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A 34-year-old man who was positive for the human immunodeficiency virus was admitted to the hospital with intense anemia and hemodynamic instability. His hemoglobin concentration at admission was 1 g/dL,… Click to show full abstract

A 34-year-old man who was positive for the human immunodeficiency virus was admitted to the hospital with intense anemia and hemodynamic instability. His hemoglobin concentration at admission was 1 g/dL, and he had a mean corpuscular volume of 110 fL/cell and an absolute reticulocyte count of 12,000/mm. He received urgent transfusion of 5 units of Images of the Monocyte Monolayer Assay performed to picture the autoimmune RBC destruction. (A) Negative control. (B,C) The patient’s results reveal that the RBCs gathered inside the macrophages in an aspect resembling a pearl necklace.

Keywords: massive autoimmune; monocyte monolayer; autoimmune hemolysis; monolayer assay; hemolysis documented

Journal Title: Transfusion
Year Published: 2018

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