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Hemolysis in the plasma of a specimen? Add a drop to clarify it

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A 38-year-old woman, with history of aplastic anemia, was admitted to the hospital with a 3-week history of epigastric pain and weight loss. A transfusion request was received at the… Click to show full abstract

A 38-year-old woman, with history of aplastic anemia, was admitted to the hospital with a 3-week history of epigastric pain and weight loss. A transfusion request was received at the transfusion department accompanied with an EDTA-anticoagulated blood sample. After centrifugation the plasma supernatant showed a reddish-brown color (see figure, left—on the right is an EDTA tube of the patient after centrifugation, and on the left, a normal sample for comparison). A new sample was requested that showed the same characteristics. We did not observe any significant interference in the pretransfusion tests. In gel card we could interpret perfectly the ABO/D groups and the irregular antibodies screening The treating physicians were contacted due to concern for hemolysis. They stated that the patient was asymptomatic and ready for discharge. The patient had normal values for LDH, bilirubin, and haptoglobin. No hemoglobinuria was detected. After ruling out hemolysis, other possible etiologies were explored. Her medication was [Correction added on Aug 21, 2020 after first online publication: author's first name & family name were swapped.] Received: 13 November 2019 Revised: 5 June 2020 Accepted: 7 June 2020

Keywords: plasma specimen; specimen add; hemolysis; hemolysis plasma; add drop; plasma

Journal Title: Transfusion
Year Published: 2020

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