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A freshly drawn blood sample was used for DAT with a commercial card (ID-Card DC-Screening II, Bio-Rad) in conditions recommended by the manufacturer. Routine serological testing was carried out with… Click to show full abstract
A freshly drawn blood sample was used for DAT with a commercial card (ID-Card DC-Screening II, Bio-Rad) in conditions recommended by the manufacturer. Routine serological testing was carried out with an automated erythrocyte magnetized technology (DuoLys kit with the QWALYS 3 EVO technology, Diagast). After informed consent, genomic DNA was isolated from peripheral blood by a semi-automated method (Maxwell 16 Blood DNA Kit in the Maxwell 16 Instrument, Promega) and eluted in molecular-grade water. Genotyping was performed successively by 1/ a commercial platform (HEA, RHCE, and RHD BeadChips, Immucor), 2/ direct sequencing of the RH exons, and 3/ quantitative multiplex PCR of short fluorescent fragments (QMPSF) in RHD and RHCE for identification of single nucleotide variations (SNVs) and structural variants (SVs), respectively.
Journal Title: Transfusion
Year Published: 2022
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