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Polymorphism of glutathione S-transferase P1 of dogs with mammary tumors.

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Mammary tumors constitute more than half of neoplasms in female dogs from different countries. Genome sequences are associated with cancer susceptibility but there is little information available about genetic polymorphisms… Click to show full abstract

Mammary tumors constitute more than half of neoplasms in female dogs from different countries. Genome sequences are associated with cancer susceptibility but there is little information available about genetic polymorphisms of glutathione S-transferase P1 (GSTP1) in canine cancers. The aim of this study was to find single nucleotide polymorphisms (SNPs) in GSTP1 of dogs (Canis lupus familiaris) with mammary tumors compared to healthy dogs and to determine the association between GSTP1 polymorphisms and the occurrence of these tumors. The study population included 36 client-owned female dogs with mammary tumors and 12 healthy female dogs, with no previous diagnosis of cancer. DNA was extracted from blood and amplified by PCR assay. PCR-products were sequenced by Sanger method and analyzed manually. The 33 polymorphisms were found in GSTP1: 1 coding SNP (exon 4), 24 non-coding SNPs (9 in exon 1), 7 deletions and 1 insertion. The 17 polymorphisms have been found in introns 1, 4, 5, and 6. The dogs with mammary tumors have significant difference from healthy in SNPs I4 c.1018+123 T>C (OR 13.412, 95%CI 1.574-114.267, P=.001), I5 c.1487+27 T>C (OR 10.737, 95%CI 1.260-91.477, P=.004), I5 c.1487+842 G>C (OR 4.714, 95%CI 1.086-20.472, P=.046) and I6 c.2481+50 A>G (OR 12.000, 95%CI 1.409-102.207, P=.002). SNP E5 c.1487 T>C and I5 c.1487+829 delG also differed significantly (P=.03) but not to the confidence interval. The study, for the first time, showed a positive association of SNPs in GSTP1 with mammary tumors of dogs, that can possibly be used to predict the occurrence of this pathology.

Keywords: female dogs; glutathione transferase; dogs mammary; mammary tumors; polymorphism glutathione

Journal Title: Veterinary and comparative oncology
Year Published: 2023

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