LAUSR

ABSTRACT Background Snakebite envenomation is a worldwide public health issue, particularly relevant to low and middle‐income countries. Bothrops spp. antivenom is needed for snakebite treatment, which requires the maintenance of healthy snakes in captivity. Hematology and clinical biochemistry are important tools for monitoring the health status of these animals, as well as detecting hemoparasites (i.e., Hepatozoon spp.). Objectives This study aims to establish hematologic and biochemical reference intervals in a population of captive B. jararaca in a Brazilian serpentarium and assess blood cell morphology. Methods Blood samples of 32 specimens of captive B. jararaca were collected and analyzed for complete blood count and biochemical variables, such as total proteins, albumin, cholesterol, triglycerides, calcium, phosphorus, urea, creatinine, uric acid, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma‐glutamyl transferase, and creatine kinase. Blood smears were also evaluated to investigate blood parasites. Each variable was statistically analyzed according to the ASVCP guidelines and compared between sexes and the occurrence of Hepatozoon spp. Results This study establishes the hematology and clinical biochemistry reference intervals and describes B. jararaca blood cell morphology. We observed no differences between sexes. Conclusions Our study provides the first reference intervals for hematologic and biochemical variables for B. jararaca on the basis of ASCVP recommendations. The RI described could be essential for the management and treatment of B. jararaca kept under similar captive conditions.