LAUSR

What is the central question of this study? The aim was to investigate whether complementary assessment of non‐invasive ultrasound imaging together with closed chest‐derived intracardiac pressure–volume catheterization is applicable to mice for an in‐depth characterization of right ventricular (RV) function even upon maintained pressure overload. What is the main finding and its importance? Characterization of RV function by the complementary use of echocardiographic imaging together with pressure–volume catheterization reveals ventricular–arterial decoupling upon maintained pressure overload, where RV systolic function correlates with ventricular–arterial coupling rather than contractility, whereas diastolic function correlates well with RV diastolic pressure. This combined approach allows us to phenotype RV function and dysfunction better in genetically modified and/or pharmacologically treated mice.