Photo by neom from unsplash
Abstract. The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The… Click to show full abstract
Abstract. The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are considered. Different contrast mechanisms, including use of nanoparticles, are discussed. Two new promising techniques that can be applied to multiphoton microscopy are described.
Journal Title: Journal of Biomedical Optics
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!