LAUSR

Abstract. In a gene sequencing system, ensquared energy is an important parameter for base-calling software. Nevertheless, the displacement between wafer and camera, which is caused by vibration and position error of the wafer stage while photographing, will decrease the image quality and further influence the ensquared energy. Our study established the relationship model between the dynamic factors and ensquared energy. Several experiments were carried out on the sequencing platform. The results revealed that the ensquared energy has a linear relationship with the location standard deviation of dynamic errors. In the sequencing platform described, if the ensquared energy value required is larger than 65%, the location standard deviation should be controlled below 140 nm. Furthermore, the ensquared energy calculated by a DNA nanoballs image is accordant with the conclusion in the meantime. The performance parameters of the wafer stage and vibration suppression could be drawn up reasonably.