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Pulsed illumination phase-contrast microscopy

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Abstract. With high spatial but low temporal coherent nanosecond (ns) green light, ns pulsed illumination phase-contrast microscopy is successfully constructed. The pulsed illumination phase-contrast microscopy can provide ns time resolution… Click to show full abstract

Abstract. With high spatial but low temporal coherent nanosecond (ns) green light, ns pulsed illumination phase-contrast microscopy is successfully constructed. The pulsed illumination phase-contrast microscopy can provide ns time resolution to resolve transient dynamics of transparent targets with high contrast ratio that cannot be achieved with traditional bright-field or phase-contrast microscopies. Transient dynamics of laser-induced microcavitation is successfully recorded with this imaging technique to demonstrate its capabilities. In addition to tracing the evolutions of vapor bubbles and shockwaves during a laser-induced microcavitation, this imaging technique can sense the refractive index change of the transparent target during a pressure field variation.

Keywords: microscopy; illumination phase; pulsed illumination; phase contrast; contrast

Journal Title: Optical Engineering
Year Published: 2020

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