LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

PABPN1 functions as a hub in the assembly of nuclear poly(A) domains that are essential for mouse oocyte development

Photo from wikipedia

Growing oocytes store a large amount of maternal mRNA to support the subsequent “maternal-zygotic transition” process. At present, it is not clear how the growing oocytes store and process the… Click to show full abstract

Growing oocytes store a large amount of maternal mRNA to support the subsequent “maternal-zygotic transition” process. At present, it is not clear how the growing oocytes store and process the newly transcribed mRNA under physiological conditions. In this study, we report non–membrane-bound compartments, nuclear poly(A) domains (NPADs), as the hub for newly transcribed mRNA, in developing mouse oocytes. The RNA binding protein PABPN1 promotes the formation of NPAD through its N-terminal disordered domain and RNA-recognized motif by means of liquid phase separation. Pabpn1-null growing oocytes cannot form NPAD normally in vivo and have defects in stability of oocyte growing–related transcripts and formation of long 3′ untranslated region isoform transcripts. Ultimately, Pabpn1fl/fl;Gdf9-Cre mice are completely sterile with primary ovarian insufficiency. These results demonstrate that NPAD formed by the phase separation properties of PABPN1-mRNA are the hub of the newly transcribed mRNA and essential for the development of oocytes and female reproduction.

Keywords: hub; poly domains; nuclear poly; mouse; pabpn1

Journal Title: Science Advances
Year Published: 2022

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.