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Genome Sequencing The RNA-guided endonuclease Cas9 has brought about a revolution in genetic engineering and genome editing. However, issues with the system include the off-target activity of the endonuclease and… Click to show full abstract
Genome Sequencing The RNA-guided endonuclease Cas9 has brought about a revolution in genetic engineering and genome editing. However, issues with the system include the off-target activity of the endonuclease and promiscuous cleavage, making accuracy in vivo a challenge. Dagdas et al. used smFRET (single-molecule Forster resonance energy transfer) to gain a better molecular understanding of this process, which should enhance the specificity of DNA targeting by Cas9. Sci. Adv. 10.1126/sciadv.aao0027 (2017).
Journal Title: Science
Year Published: 2017
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