The influence of glutamate dehydrogenase activity on the production of L-proline in the producer strain Brevibacterium flavum AP111 was studied. Transformants of the Brevibacterium flavum strain AP111 were obtained with… Click to show full abstract
The influence of glutamate dehydrogenase activity on the production of L-proline in the producer strain Brevibacterium flavum AP111 was studied. Transformants of the Brevibacterium flavum strain AP111 were obtained with the use of a recombinant plasmid previously constructed by us, pVOG10. It includes the encoding sequence of the gdh gene, which is responsible for the synthesis of glutamate dehydrogenase. The production of L-proline in transformants was shown to have increased by more than 40%. The results can serve as the basis for the use of this approach to improve strains producing other amino acids of the glutamine family.
               
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