LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Crystallization and X-Ray Diffraction Studies of Salmonella Invasion Protein D (SipD), Insight Tip Component of Salmonella typhi Type III Secretion System

Photo from wikipedia

Many pathogenic gram-negative bacteria including Salmonella typhi utilize type III secretion systems (T3SSs) to inject bacterial proteins into host cells and to initiate infections. One of the essential components of… Click to show full abstract

Many pathogenic gram-negative bacteria including Salmonella typhi utilize type III secretion systems (T3SSs) to inject bacterial proteins into host cells and to initiate infections. One of the essential components of the T3SS is the tip protein known as SipD, translocon component via the type III secretion system SPI-1. Here, recombinant SipD is produced and crystallized; the details of cloning, expression, and crystallization are reported. Crystals of SipD are obtained by sitting-drop vapour diffusion method. X-ray diffraction data are collected up to 3.8 A resolution. Single crystals belong to the monoclinic sp. gr. P2; unit-cell parameters: a = 70.86, b = 103.70, c = 89.28 A, β = 107.63°.

Keywords: salmonella typhi; iii secretion; type iii

Journal Title: Crystallography Reports
Year Published: 2019

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.