LAUSR

Background: The activation of self-specific T cells is essential in pathogenesis of Takayasu arteritis (TAK). Dendritic cell (DC) plays an indispensable role as the only antigen presenting cell for initial T cell, and Toll-like receptors (TLRs) are common source of activation signals for DCs. Then we speculate that there are activation of TLRs in TAK patients. Objectives: To investigate the activation of TLRs in TAK patients. Methods: Twenty-seven TAK patients were enrolled during April to October in 2019, with diagnosis met the 1990 criteria of American College of Rheumatology. Patient were divided into groups by the disease activity and medication history. Disease activity was assessed by the 1994 NIH criteria. Quantitative Real-time Polymerase Chain Reaction (RT-qPCR) was used to analyze the mRNA relative abundance of 28 target genes in peripheral blood mononuclear cells (PBMCs). Differences between groups and correlation between any two genes were analyzed. Results: The demographic data and clinical features of TAK patients were shown in Table 1. (1) Compared with health control (HC) group, mRNA abundance of TLR2, TLR4, P50, P65, IκBα, CTLA4, CD3, and BCL6 in untreated TAK group was upregulated ( Conclusion: TLRs-NFκB pathway may be activated in TAK patients, with upregulation of BCL6, and there may be deficiency of CD40. TLR2, TLR4, PD-1, LAG3, CD40 and BCL6 may play roles in the pathogenesis of TAK. p50, CD28, TCR, GATA3, RCRC and FOXP3 may be related to the disease activity of TAK. Disclosure of Interests: Yixiao Tian: None declared, Jing Li: None declared, Xinping Tian: None declared, Xiaofeng Zeng Consultant of: MSD Pharmaceuticals