LAUSR

Introduction Non-coding RNAs are become one of the most investigated biomarkers in non-invasive diagnostics of cancer diseases. In this study we explore the potential of three miRNAs and one lncRNA as biomarkers in advanced laryngeal cancer (LSCC). Material and methods Plasma samples were obtained from 22 patients with advanced LSCC and 21 healthy controls. Relative expression of miR-145–5 p, miR-196a-5p, miR-222–3 p and lncRNA MALAT1 was performed with RT-qPCR. Data was analysed by SPSS 17.0. A p-value less than 0.05 was considered statistically significant. Results and discussions Our data showed significant ectopic expressions of miR-145–5 p (p=0.022), miR-222–3 p (p=0.013), miR-196a-5p (p=0.055), and lncRNA MALAT1 (p=0.004) in LSCC patients. Decreased expression levels of miR-145–5 p were obtained in 16 patients (mean RQ=0.828;SD=1.42,n=22), miR-222–3 p in 13 patients (mean RQ=0.59;SD=0.88;n=22) and lncRNA MALAT1 in 9 patients (mean RQ=0.67;SD=0.42;n=22). Upregulated levels of miR-196a-5p were reach in 17 patients (mean RQ=2.99;SD=1.43;n=22). Association analysis between markers and TNM stage, differentiation, tumour localization, tobacco and alcohol use was performed. Significant relation between lncRNA MALAT1 with nodal metastasis (p=0.049) and differentiation stage (p=0.020) were reached, as highest expression levels were obtained in those patients with nodal metastasis and poor differentiation tumour. None of the miRNAs showed significant association with any of clinicopathological characteristics. In order to evaluate the potential of included in the study markers as discriminative markers for LCSS patients, we used ROC analysis. The strongest significant potential was combination of miR-145–5 p, miR-196a-5p and lncRNa MALAT1 with AUC=0.870 (p=0.0001,95%CI:0.758–0.982; 86.4% sensitivity and 71.4% specificity). Separately, the ROC results showed for miR-222–3 p: AUC=0.703 (p=0.022,95%CI:0.547–0.860), miR-145–5 p: AUC=721(p=0.013 95% CI:0.570–0.872), miR-196a-5p: AUC=0.671 (p=0.055, 95% CI:0.4860.856), and lncRNA MALAT1: AUC=0.753 (p=0.004, 95% CI: 0.601–0.905). Conclusion Overexpression of included in the study miRNAs and lncRNA might take role in cancerogenesis of LSCC. In addition, lncRNA MALAT was associated with advanced tumorogenesis. The fusion of miR-145–5 p, miR-196a-5p and MALAT1 could be potential marker for non invasive diagnostic of LSCC. Grants:This work was supported by Project№ 8553/12.12.2016/Contract №D-137/MU-Sofia; DUNK01/2/2009/NSF.