LAUSR

Introduction/Background HPV infection is strongly associated to cervical cancer. knowledge of the HPV genotypes distribution is requires before vaccination. Aim To investigate the HPV prevalence and genotype distribution associated with cervical lesions from women attending the Ibn Rochd Universitary Hospital in Casablanca. Methodology A cross-sectional study was conducted and completed with a standardized questionnaire on 121 women aged 21 to 72 years. HPV infection was assessed by nested-PCR using MY09/MY11 and GP5+/GP6+ primers, and direct sequencing. Multiple infections were assessed by type-specific PCR for HPV 6, 11, 16, 18, 31 and 33. Results HPV-DNA was detected in 41.1% (95%CI, 35.8–46.5) of the study population. HR-HPV were found in 84.8% of positive samples, of which 15.9% involved multiple-type infections. The five commonest genotypes were HPV16, HPV33, HPV70, HPV18 and HPV31. The prevalence of HPV infection increased with the severity of cervical lesions from 11.7% in N/BRCC to 100% in HSIL/ICC. HPV infection was significant associated with age, number of sexual partners, number of pregnancy, education level and first sexual intercourse age (p<0.05). Conclusion Based on the high prevalence of HPV infection found in this study, the necessity of cervical cancer prevention program in Morocco is entirely justified. Disclosure Nothing to disclose.