Abstract: This study investigates the effect of two dietary fats, namely linseed oil (LO) and lard (LF), and supplementation with pumpkin seeds (PS) on the performance, oxidative status, and blood… Click to show full abstract
Abstract: This study investigates the effect of two dietary fats, namely linseed oil (LO) and lard (LF), and supplementation with pumpkin seeds (PS) on the performance, oxidative status, and blood serum metabolites of broilers, along with the composition, oxidative stability, and fatty acid (FA) profile of broiler meat. Ninety-six 64-d-old naked neck chickens were randomly distributed into four groups of 24 chickens each (8 chickens per cage, three cages per treatment) and fed a LO diet, a LO diet supplemented with 50 g PS kg-1, a LF diet, and a LF diet supplemented with 50 g PS kg-1. The antioxidant system is increased and reactive oxygen species decreased in chickens fed PS diets. Glycemia and cholesterol levels were significantly lower in the chickens fed PS diets than in those given diets without supplementation; the cholesterol levels were also significantly lower in the chickens fed LO diets than those fed LF diets. However, the LO diet affected the fresh meat quality by decreasing pH values of the meat at 24 h after slaughter. Adding PS to the LO diet improved the oxidative stability of the muscle at day 3 of refrigerated storage. The use of LO instead of LF favourably modulates the FA profile of meat, by increasing both linoleic and α-linolenic acid content and reducing the n-6/n-3 polyunsaturated FA ratio, atherogenic and thrombogenic index.
               
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