LAUSR

Bovine serum albumin (BSA) emerged as a potential bioprotein in the sensing field. Understanding the interactions of BSA with the analyte biomolecule has not been much reported. Herein, we report the functionally modified BSA (f-BSA) and its consecutive adsorption onto DNA for the ultrasensitive protein sensing. The DNA-modified f-BSA (DNA@f-BSA) composite was further characterized by using scanning electron microscopy, X-ray diffraction, and Fourier transform-infrared spectroscopy. Obtained results demonstrated excellent composite formulation due to electrostatic interaction/binding intercalation and provided promising scaffold for protein biosensor. The as-prepared DNA modified f-BSA (DNA@f-BSA) based biocomposite can be used as a selective probe for the detection of BSA by recording square wave voltammetry signals. The sensitivity of the hybrid biosensor significantly improved the BSA detection over a wide concentration range from 1×10-20 g/mL to 1×10-4 g/mL with a detection limit of 2.18×10-21 g/mL. Thus, the results have revealed the promising biosensor interactions which pave the way for the sensitive protein detection.