LAUSR

Hepatocellular carcinoma (HCC) is one of the most frequent and fatal cancers. However, traditional clinical imaging modalities cannot detect small tumors and are not suitable for the early stage diagnosis of hepatic cancer. In this study, we applied silicon nanowire field-effect transistors (SiNW-FETs) as a biosensor to detect α-fucosidase (AFU), which is a biomarker of high sensitivity and specificity for the HCC diagnosis. Fuconojirimycin (FNJ) is an inhibitor of AFU with very high binding affinity to AFU. By modifying the FNJs of optimized receptor length and number density on SiNW-FET (referred to as FNJ/SiNW-FET), this biosensing device can detect AFU to a very low concentration level with the detection limit of ∼1.3 pM. With the merits of high sensitivity, target specificity, and label-free detection, this FNJ/SiNW-FET can be developed as a powerful biosensor to detect AFU for the early HCC diagnosis. © The Author(s) 2018. Published by ECS. This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives 4.0 License (CC BY-NC-ND, http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is not changed in any way and is properly cited. For permission for commercial reuse, please email: [email protected]. [DOI: 10.1149/2.0231807jss]