LAUSR

The enteric nervous system comprises millions of neurons and glia embedded in the wall of the gastrointestinal tract. It not only controls important functions of the gut, but also interacts with the immune system, gut microbiota and the gut-brain-axis, thereby playing a key role in health and disease of the whole organism. Any disturbance of this intricate system is mirrored in an alteration of electrical functionality, making electrophysiological methods important tools for investigating ENS-related disorders. Microelectrode arrays provide an appropriate non-invasive approach of recording signals from multiple neurons or whole networks simultaneously. However, studying isolated cells of the ENS can be challenging, considering the limited time that these cells can be kept vital in vitro. Therefore, we developed an alternative approach cultivating cells on glass samples with spacers (fabricated by photolithography methods). The spacers allow the cells to grow upside-down in a spatially confined environment, while enabling acute consecutive recordings of multiple ENS-cultures on the same MEA. Upside-down culture also shows beneficial effects on growth and behavior of enteric neural cultures. The number of dead cells was significantly decreased, neural networks showed higher resemblance to the myenteric plexus ex vivo, while producing more stable signals than cultures grown in the conventional way. Overall, our results indicate that the upside-down approach not only allows to investigate the impact of neurological diseases in vitro but could also offer insights into growth and development of the ENS under conditions much closer to the in vivo environment.