• Targeted RNA-Seq of expression markers in NSCLC FFPE tumors requires integrated reagents, protocols, and interpretive software that can ensure consistent results across laboratories. • In this study, a comprehensive… Click to show full abstract
• Targeted RNA-Seq of expression markers in NSCLC FFPE tumors requires integrated reagents, protocols, and interpretive software that can ensure consistent results across laboratories. • In this study, a comprehensive targeted NGS system, the QuantideX® NGS RNA Lung Cancer Kit (RUO)*, was evaluated in 5 laboratories to assess accuracy and reproducibility. • The results demonstrated excellent agreement among laboratories for the detection of targeted fusions, 3’-5’ imbalances, and MET exon 14 skipping events, with actionable fusions reliably detected at <10 ng FFPE RNA.
               
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