LAUSR

Introduction: In this study, we aimed to develop a versatile in vivo model of prostate cancer using orthotopic xenografts, which adequately represents intraprostatic tumor growth as well as the natural routes of metastatic spread. Materials and Methods: 5x10 5 VCaP-, LuCaP136-, and LuCaP147 cells were injected into the prostate of male CB17-SCID mice (n=8 for each cell line; VCaP cultured as monolayer, LuCaP136 and LuCaP147 as three-dimensional spheroid culture). During 12 weeks of follow-up, orthotopic tumor growth and the development of metastases was monitored by repetitive serum-PSA measurements and imaging studies (ultrasonography, CT, MRI). At autopsy, primary tumors and metastases were harvested and examined by conventional histology (HE 2017 Sep 24-27; Orlando, Florida. Philadelphia (PA): AACR; Cancer Res 2018;78(10 Suppl):Abstract nr B35.