LAUSR

The inflammatory response following acute ischemic stroke is a well-known and widely studied phenomenon, but the mechanism is still unclear. After ischemic stroke, microglia and recruited macrophages play major roles in neuroinflammation after ischemic stroke. To characterize these roles, we explored how these cells affect counterpart’s differentiation, polarization and infiltration or migration. Methods: BV2 (microglia cell line) were treated with lipopolysaccharides (LPS; 10ng/mL) or interleukin-4 (IL-4; 25ng/mL), and the supernatant was collected as M1 or M2 conditioned media of BV2. The supernatant of PMA differentiated THP-1 (monocyte cell line) followed by LPS (20ng/mL) treatment or interleukin-13 (IL-13; 25ng/mL) & IL-4(25ng/mL) co-treatment was collected as M1 or M2 conditioned media of THP-1. After BV2 or THP-1 cultured in conditioned media, the activation and polarization were assessed by ICC and confocal microscopy for CD11b, CD86 and CD206. Transwell inserts of 3 and 8 μm pore membrane were us...