In this study, the conditions for establishment of a primary cell culture of haemocytes from the Australian red claw crayfish, Cherax quadricarinatus, were investigated. Five commercial media were assessed for… Click to show full abstract
In this study, the conditions for establishment of a primary cell culture of haemocytes from the Australian red claw crayfish, Cherax quadricarinatus, were investigated. Five commercial media were assessed for haemocyte viability: Leibovitz L-15 medium (L-15), modified Leibovitz L-15 medium (ML-15, 2 × concentration), Grace’s insect medium, DMEM medium, and I-max medium. In addition, L-15 and Grace’s insect medium supplemented with 10%, 15%, and 20% charcoal-dextran-treated foetal bovine serum (FBS) were assessed for their effects on haemocyte growth. The optimal culture system was established as follows: L-15 basal medium with the addition of 0.4 g/l glucose, 2 g/l NaCl, 10% FBS, 100 U ml⁻¹ penicillin, 100 μg ml⁻¹ streptomycin, and pH 7.2-7.4. In addition, Giemsa staining of the cultured haemocyte monolayer revealed that two types of cell could be observed: semigranular cells (SGC) and granular cells (GC). Our study could enable further investigation of the immune function in this species.
               
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