LAUSR

Heavy metals and metalloids originating from industrial, agricultural, and urban wastes and increasing in aquatic ecosystems cause genotoxic damage to fish species. This study aimed to determine the potential genotoxic effects of mixtures of aluminum, arsenic, and manganese in Cyprinus carpio. The effects of the mixtures on erythrocyte cells of C. carpio were examined using the comet assay, micronucleus test, and erythrocytic nuclear abnormalities in two groups after exposure to three doses of the mixtures (Group A; Dose 1: 0.3 + 0.1 + 0.02 mg/L, Dose 2: 0.6 + 0.2 + 0.04 mg/L, Dose 3: 0.9 + 0.3 + 0.06 mg/L and Group B; Dose 1: 1 + 3 +0.1 mg/L, Dose 2: 2 + 6 + 0.2 mg/L, Dose 3: 3 + 9 + 0.3 mg/L). Experimental groups were formed according to the permissible limits specified in the Turkish Surface Water Quality Regulation (TSWQR). The results of comet assay parameters such as tail DNA %, tail moment, and olive tail moment confirmed the genotoxic effect of metal(oid)s mixtures on erythrocyte cells compared with control groups and showed that DNA damage increased with increasing the concentrations. The micronucleus and other nuclear abnormalities such as blebbed nuclei, notched nuclei, eightshaped nuclei, lobed nuclei, and binucleated cells were detected in the erythrocyte cells exposed to the mixtures. Consequently, it was found that the frequency of micronucleus and erythrocytic nuclear abnormalities significantly increased in the erythrocyte cells exposed to metal(oid) concentrations compared to control groups. These results show the existence of potential genotoxicity in C. carpio even at the minimum values specified in the TSWQR after exposure to the mixtures.