LAUSR

The use of nanotechnology in the agrochemical industry has become increasingly popular over the past decade, raising the question of whether these products may represent a risk or benefit compared to their conventional presentations. In this study, we aimed to evaluate the different genotoxic effects of the Complete encapsulated presentation (CEP), the micro encapsulated fraction (MEF), and the nano encapsulated fraction (NEF) of two pesticides (Karate® and Ampligo®) in lymphocytes from human peripheral blood. To test the different fractions, the pesticides were separated by centrifugations by the average size of the capsule, then were characterized by the general composition of the capsule by RAMAN and FTIR spectroscopy and the active ingredient of both pesticides by gas chromatography-mass spectrometry. Each fraction was tested separately and analyzed by comet assay through the tail moment and the percentage of DNA in the tail and the cytokinesis-block micronucleus through their frequency of micronucleus, nucleoplasmic bridges, and nuclear buds. The nuclear division index and the Nuclear Division Cytotoxicity Index were also measured. For both pesticides, the CEP increased the genetic damage observed in the tail moment and percentage of DNA in the tail at all concentrations for both pesticides. However, in the micronucleus test, NEF induced more micronuclei than MEF and CEP in all treatments reducing cell proliferation as the concentration decreased for both pesticides. These results suggested that NEF had more genotoxic effects in both pesticides, increasing the damage to the cells.