LAUSR

Figure 3. Overexpression of WDFY2 suppressed the colony formation and migration ability of prostate cancer cells. (a) Western blot showed the establishment of WDFY2 stably expressed cell lines in DU145 and PC-3. (b) Representative results of a migration assay for DU145 and PC-3 cells transfected with control vector or WDFY2 (scale bars=100mm). (c) Quantitation of the migration of DU145 and PC3 cells transfected with control vector and WDFY2. (d) Results of wound-healing assay of the DU145 and PC-3 cells transfected with control vector and WDFY2. Each cell culture monolayers was scratched with a white micropipette tip, and then, the cell images were taken at the indicated time points. (e) Quantitation of migration ratio of DU145 and PC-3 cells transfected with control vector and WDFY2. (f) Results of colony-formation assay in DU145 and PC-3 cells transfected with control vector and WDFY2. (g) Quantitation of clones for DU145 and PC3 cells transfected with control vector and WDFY2.