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BACKGROUND Having meaning in life is a protective factor for psychological well-being. Accurate assessment of this construct needs a valid and reliable tool. PURPOSE This study aims to evaluate the psychometric properties of the Farsi version of the meaning of life questionnaire in patients with cancer. METHOD In this cross-sectional study, after translating the questionnaire to Farsi, in a sample of 212 patients with cancer, feasibility, content and convergent validity, exploratory and confirmatory factor analysis, internal consistency, stability, and responsiveness were evaluated. RESULTS The results show that the content validity ratio of all ten items was greater than 0.49. Also, the modified Kappa coefficient of each item was greater than 0.6. The maximum likelihood exploratory factor analysis extracted one factor, which explains 76.13% of the total variance of the sample. Item nine was removed. The confirmatory factor analysis results show that the one-factor model had good fit indices. The Cronbach's alpha, McDonald's omega, composite reliability, MaxR, and intraclass correlation coefficient were 0.96, 0.96, 0.96, 0.96, and 0.98, respectively. The questionnaires had responsiveness and its response time was 3 s. CONCLUSION AND POLICY SUMMARY The nine-item Farsi version of the meaning of life questionnaire has good validity and reliability and responsiveness.