LAUSR

p=0.066 * D WT p62 Figure 4. Mitochondrial functions in p622/2leukemia cells were defective. (A) Mitochondrial superoxide levels of LK HSC cells from healthy mice (n 5 3 per group) and GFP LK blasts from MN1 leukemic mice (n5 8 in WT and n5 14 in p622/2 leukemic mice) were analyzed by flow cytometry using MitoSOX. (B) Pearson’s correlation was used to determine the correlation between the proportions of MitoSOX leukemic cells with the survival time of leukemic mice. (C) Mitochondrial respiration of WT and p622/2 MN1 leukemic mice (n 5 3 per group) was determined by measuring the OCR. The experiment was performed in real time by the 96-well Seahorse Bioscience Extracellular Flux Analyzer XF96. (D) The rates of basal respiration, maximal respiration, and adenosine triphosphate (ATP) production were compared between WT and p622/2 group (n 5 3 per group). Values are mean 6 SEM. ns, not significant; *P # .05; **P # .01; ***P # .001.