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Inhibition of casein kinase 1δ disrupts translation initiation and exerts potent antilymphoma activity

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Cell line and reagents Mantle cell lymphoma cell lines Jeko-1, Z-138, JVM-2, MAVER-1, multiple myeloma cell lines H929 and MM1S, diffuse large B-cell lymphoma cell lines SUDHL-6 and SUDHL-10, and… Click to show full abstract

Cell line and reagents Mantle cell lymphoma cell lines Jeko-1, Z-138, JVM-2, MAVER-1, multiple myeloma cell lines H929 and MM1S, diffuse large B-cell lymphoma cell lines SUDHL-6 and SUDHL-10, and MTA cell lines FEPD and MAC2A were grown in RPMI medium with 10% fetal calf serum. MAC2A and MTA (PTCL) were grown in RPMI medium with 10% fetal calf serum and 1% sodium pyruvate. OCI-LY7 and OCI-LY10, VAL (DLBCL) SMZ1 (PTCL) cell lines were grown in Iscove modified Dulbecco medium with 10% fetal calf serum. Fresh medium was added every 2 to 3 days, and the cells were kept at a cell concentration of 0.1 to 1x 106 /mL. MDA-MB-231 and Cells were incubated at 37 oC in a 5% CO2 atmosphere, 95% humidity in CO2 incubator.

Keywords: medium fetal; cell; calf serum; fetal calf; cell lines

Journal Title: Blood Advances
Year Published: 2022

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