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Background – PBMCs need to be responsive towards systemic changes in the body and mirror the changes in their microenvironment to mount an immunological response, which depending on the metabolic… Click to show full abstract
Background – PBMCs need to be responsive towards systemic changes in the body and mirror the changes in their microenvironment to mount an immunological response, which depending on the metabolic state of the cells is either pro-inflammatory or anti-inflammatory. We aimed to understand how the metabolic state of PBMCs is altered by chronic exposure to cigarette/biomass smoke in COPD subjects. Methods: – PBMCs were isolated from 10ml of blood obtained from healthy (n=11) and moderate – severe COPD (n=7) subjects. COPD was confirmed as per GOLD guidelines using spirometry with a post-bronchodilator FEV1/FVC ratio below 0.7. The cells were plated at a density of 150,000/ well and mitochondrial respiration (Oxygen Consumption Rate, OCR) and glycolysis (ExtraCellular Acidification Rate, ECAR) was measured using the Seahorse Extracellular Flux Analyzer, (XFp, Agilent Technologies, USA). Results: and conclusion – PBMCs from COPD subjects showed a significant reduction in mitochondrial respiration (p
Journal Title: European Respiratory Journal
Year Published: 2017
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