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© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/. The Creative Commons Public Domain Dedication waiver (http:// creat iveco mmons. org/ publi cdoma in/ zero/1. 0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Editorial Research and technologies involving carbohydrates to carbohydrate-based materials and converting carbohydrates for renewable energy, agri and health fall under the umbrella of glycobiotechnology. Microbial glycobiotechnology refers to sustainable bioprocesses for the bioconversion of carbohydrates through microbial enzymes and microbial technologies and the generation of tailor-made carbohydrate bioproducts/polymers for various novel applications in bioenergy and bioenergy fuels, biomaterials, in food and nutrition, and pharmaceuticals.This special issue is intended to present a special collection with a thematic scheme: Microbial Glycobiotechnology. Eighteen articles, connected to glycan biosynthesis, glycoenzymology, modification of complex carbohydrates and their corresponding glycoconjugates, including glycans, glycoproteins, glycolipids, biological functions of glycoconjugates and glycolipids, have been published there [1–18].