LAUSR

Background Increasing evidence suggested N 6 -methyladenosine (m 6 A) modification is crucial for male germline development. However, m 6 A modification of lncRNAs gains a little attention in amphibians in recent years. Xenopus laevis ( X. laevis ) was chosen to be an ideal model organism for testing environmental endocrine disrupting chemicals (EDCs) exposure and resultant effects. Atrazine (AZ) as an endocrine disrupt can effect development of testis in amphibians. Our previous study revealed that m 6 A is a highly conserved modification across the species. Results The results of m 6 A sequences showed that m 6 A-methylated lncRNAs enriched in intergenic region in testes of X. laevis . We further examined the differential expression of lncRNAs m 6 A sites in testes of AZ-exposed and compared with that in animals from control group. The results indicated that up to 198 differentially methylated m 6 A sites were detected within 188 lncRNAs, in which 89 significantly up-methylated sites and 109 significantly down-methylated sites. Data from KEGG pathway analysis indicated that AZ-affected lncRNAs m 6 A sites were mainly involved in 10 pathways in which 3 mutual pathways were found in the result of differentially m 6 A-methylated mRNAs. Conclusions These findings suggested that differentially m 6 A-methylated lncRNAs and these 3 pathways may act on regulatory roles in abnormal testis development of AZ-exposed X. laevis . This study for the first time provides insights into the profile of lncRNAs m 6 A modifications in amphibian species.