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BACKGROUND Cytokine-induced killer (CIK) cells are ex-vivo expanded T cells which present a phenotype of both T and Natural Killer cell properties. OBJECTIVE To compare the proliferation and functional properties… Click to show full abstract
BACKGROUND Cytokine-induced killer (CIK) cells are ex-vivo expanded T cells which present a phenotype of both T and Natural Killer cell properties. OBJECTIVE To compare the proliferation and functional properties of human CIK cells cultured in three cell culture plasticwares. METHODS The number and viability of CIK cells were monitored. The expression of surface markers (CD3 and CD56), TH1 cytokines (IFN-γ and TNF-α), and cytolytic granules (granzyme B and perforin) were determined by flow cytometry. RESULTS The number of CIK cells cultured in a static bag was highest compared to those in a petri dish and gas-permeable flask. However, CIK cells cultured in all plasticwares similarity expressed surface marker, TH1 cytokines, and cytolytic granules. CONCLUSIONS Considering safety, efficacy, and cost, a static bag is the best plasticware for culturing CIK cells.
Journal Title: Asian Pacific journal of allergy and immunology
Year Published: 2020
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