LAUSR

Salim MA, Budi RSW, Setyaningsih L, Iskandar, Wahyudi I, Kirmi H. 2020. Root colonization by arbuscular mycorrhizal fungi (AMF) in various age classes of revegetation post-coal mine. Biodiversitas 21: 5013-5022. This study aims to evaluate the status of colonization of the roots of the host plant in various age classes of revegetation of post-coal mining land associated with AMF spores populations and soil fertility. The study was conducted in the post-mining revegetation land at PT. Berau Coal, East Kalimantan. Isolation of the spores was carried out using the wet-sieving and centrifugation method, while the colonization of the roots was done by the root coloring technique. Result showed that revegetation activities were able to increase the content of some nutrients in the soil. The AMF population showed tendency an increase along with the increasing revegetation age classes. Eight-year revegetation age classes had the highest average number of spores. Plant species and understorey have shown the existence of AMF colonization with different values for each species. AMF root colonization had a negative relationship with the number of spores in the soil. The C-organic, N-total, CEC Ca, Mg and Fe contents had a positive correlation with AMF root colonization, while available P, total P, K, and Al had a negative correlation with AMF root colonization. Mg was significantly correlated (r = 0.861) to AMF root colonization.