We use non-degenerate two-photon excitation where the two excitation beams are displaced in space outside the focal volume to increase the signal-to-background ratio (SBR), overcoming the fundamental penetration depth limit… Click to show full abstract
We use non-degenerate two-photon excitation where the two excitation beams are displaced in space outside the focal volume to increase the signal-to-background ratio (SBR), overcoming the fundamental penetration depth limit of conventional two-photon microscopy. © 2020 The Author(s) OCIS codes: (180.0180) Microscopy; (180.2520) Fluorescence microscopy; (180.4315) Nonlinear microscopy.
               
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