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Single-path single-shot phase-shifting digital holographic microscopy without a laser light source.

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We propose single-path single-shot phase-shifting digital holographic microscopy (SSP-DHM) in which the quantitative phase information of an object wave is acquired without a laser light source. Multiple phase-shifted holograms are… Click to show full abstract

We propose single-path single-shot phase-shifting digital holographic microscopy (SSP-DHM) in which the quantitative phase information of an object wave is acquired without a laser light source. Multiple phase-shifted holograms are simultaneously obtained using a linear polarizer, a liquid crystal on a silicon spatial light modulator (LCoS-SLM), and a polarization-imaging camera. Complex amplitude imaging of a USAF1951 test target and phase imaging of transparent HeLa cells are performed to show its quantitative phase-imaging ability. We also conduct an experiment for the motion-picture imaging of transparent particles to highlight the single-shot imaging ability of SSP-DHM.

Keywords: phase; single path; microscopy; light; single shot

Journal Title: Optics express
Year Published: 2022

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