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The Chinese pine caterpillar Dendrolimus tabulaeformis is an important destructive leaf borer in boreal coniferous forests in China. This species overwinters in the larval stage. Changes in supercooling capacity and physiological-biochemical parameters of D. tabulaeformis larvae from a natural population were evaluated at different stages during the overwintering period. Cold hardiness of overwintering larvae collected in January was signifi cantly greater than that of larvae collected in other months. January larvae survived for 15 days at –10°C and for approximately 2 days at –15°C. By contrast, larvae collected in September survived for no more than 4 h at –5°C and those in November and March no more than 1 day at –15°C. Supercooling point gradually decreased from –5.9 ± 0.3°C in September to a minimum of –14.1 ± 1.0°C in November, then gradually increased to the original value with the advent of spring. Water content gradually decreased from September to November, remained at approximately 74.5% until March and then gradually increased to levels similar to those in September. The lipid content gradually decreased from September to November, remained stable at approximately 3.2% until March and then gradually increased to levels similar to those in September. Glycogen content increased to a peak in November and then decreased. The concentrations of several metabolites showed signifi cant seasonal changes. The most prominent metabolite was trehalose with a seasonal maximum in November. Glucose levels were highest in January and then gradually decreased until in May they were at levels similar to those in September. Glycerol levels remained relatively stable during winter but increased signifi cantly in May. This study indicates that D. tabulaeformis is a freeze-avoidant insect. Larvae increase their supercooling capacity by regulating physiological-biochemical parameters during overwintering. Our results provide the basis for further research into the mechanism of cold hardiness in this species. * Corresponding author; e-mail: [email protected] INTRODUCTION All stages of an insect’s life cycle are affected by temperature. Low temperature is a key factor in insect growth and development and can impose strict limits on insect populations (Jing & Kang, 2002; Wang & Kang, 2014). To minimize the risk of damage by low ambient temperatures, overwintering insects often choose protected microhabitats such as the upper layer of soil, leaf litter or under snow, where temperature remains higher than air temperature (Lee, 1989; Danks, 2006; Feng et al., 2014). Insects also can adjust physiological-biochemical parameters to increase their supercooling capacity, thus enhancing their cold hardiness (Lee, 1989; Storey & Storey, 1991). The degree of cold hardiness effects on not only the survival, reproduction and spread of an insect population, but also population density the following year (Liu, 1963; Jing & Eur. J. Entomol. 115: 198–207, 2018 doi: 10.14411/eje.2018.018