LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Fibroblast growth factor‐23 and parathyroid hormone suppress small intestinal magnesium absorption

Photo by anniespratt from unsplash

In the present study, we examined the systemic and direct effects of parathyroid hormone (PTH) and fibroblast growth factor‐23 (FGF‐23) on duodenal, jejunal, and ileal Mg2+ absorption. The rats were… Click to show full abstract

In the present study, we examined the systemic and direct effects of parathyroid hormone (PTH) and fibroblast growth factor‐23 (FGF‐23) on duodenal, jejunal, and ileal Mg2+ absorption. The rats were injected with FGF‐23 or PTH for 5 h before collecting the duodenum, jejunum, and ileum for Mg2+ transport analysis in Ussing chambers. The duodenum, jejunum, and ileum were directly exposed to FGF‐23, PTH, or FGF‐23 plus PTH with or without cell signaling inhibitors for 150 min in Ussing chambers prior to performing the Mg2+ transport study. The small intestinal tissues were also subjected to western blot analyses for FGF receptor (FGFR), PTH receptor (PTHR), Klotho, transient receptor potential melastatin 6 (TRPM6), and cyclin as well as the cystathionine β‐synthase domain divalent metal cation transport mediator 4 (CNNM4) expression. The small intestine abundantly expressed FGFR and PTHR proteins, whereas, Klotho was not expressed in rat small intestine. Systemic PTH or FGF‐23 injection significantly suppressed transcellular Mg2+ transport in the duodenum and jejunum. Direct FGF‐23‐, PTH‐, or FGF‐23 plus PTH exposure also suppressed transcellular Mg2+ absorption in the duodenum and jejunum. There was no additional inhibitory effect of PTH and FGF‐23 on intestinal Mg2+ absorption. The inhibitory effect of PTH, FGF‐23, or FGF‐23 plus PTH was abolished by Gö 6850. Systemic PTH‐ or FGF‐23‐injection significantly decreased membranous TRPM6 expression, but increased cytosolic CNNM4 expression in the duodenum, jejunum, and ileum. In the present study, we propose a novel magnesiotropic action of PTH and FGF‐23 by modulating small intestinal Mg2+ absorption.

Keywords: mg2; pth fgf; duodenum jejunum; fgf; absorption; pth

Journal Title: Physiological Reports
Year Published: 2022

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.