LAUSR

Nasal nitric oxide (nNO) levels are extremely low in Primary Ciliary Dyskinesia, and measurement of this nasal gas is recommended as a PCD diagnostic test in cooperative patients ≥5 years old. However, nNO measurements must be performed with chemiluminescence analyzers using a standardized protocol to ensure proper results, as nNO values can be influenced by various internal and external factors. Repeat nNO testing on separate visits is required to ensure low diagnostic values are persistent and consistent with PCD. This technical paper presents the standard operating procedures for nNO measurement used by The PCD Foundation's Clinical and Research Center Network at various specialty centers across North America. Adherence to this document ensures reliable nNO testing and high diagnostic accuracy when employed in a population with appropriate clinical phenotypes for Primary Ciliary Dyskinesia.