LAUSR

Abstract Comparing with other biological processes, a technological method associated with bio-energy has been developed as the result of the high possibility of fermentative hydrogen productivity; In addition, restricted fossil fuel has been also substituted. The production of fermentative hydrogen is a complicated process influenced by different factors including different kinds of micro-organisms, initial concentration of substrate, inoculum size, initial pH of the medium, concentration of nutrients metals, etc. Thus, the present study is aiming at detecting such different agents to obtain the highest yield of hydrogen and progressing its operation through improving the efficiency of these agents by reliable experimental design. Clostridium saccharoperbutylacetonicum N1-4(ATCC 13564) was applied to produce hydrogen in a batch manner. The highest yield of hydrogen has been resulted in 3.016 mol of hydrogen per mol glucose with production rate of 3.281 mole of hydrogen per hour at 37 °C, 10 % of inoculums size, 10 g/L of an initial glucose concentration, initial pH of medium of 6.5, 30 mg/L of FeSO4.7H2O. Furthermore, the experimental results were compared to kinetic model of Gompertz. Moreover, it was found out that the experimental issues were compatible with this kinetic model with the regression coefficient values of 0.989.