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ABSTRACT Weakley, J, Castilla, AP, Ramos, AG, Banyard, H, Thurlow, F, Edwards, T, Morrison, M, McMahon, E, and Owen, C. The effect of traditional, rest redistribution, and velocity-based prescription on repeated sprint training performance and responses in semi-professional athletes. J Strength Cond Res XX(X): 000-000, 2022-The aim of this study was to investigate the effects of traditional, rest redistribution, and velocity-based repeated sprint training methods on repeated sprint performance, perceived effort, heart rate, and changes in force-velocity-power (FVP) profiles in male semiprofessional athletes. In a randomized crossover design, a traditional (2 sets of 6 repetitions [TRAD]), 2 different rest redistribution (4 sets of 3 repetitions [RR4] and 12 sets of 1 repetition [RR12]), and a 5% velocity loss (VL5%) (12 repetitions, with sets terminated when a 5% reduction in mean velocity had occurred) condition were completed. Mean and peak velocity, mean heart rate, and differential ratings of perceived exertion (dRPE) were measured throughout each session, while horizontal FVP profiles were assessed presession and postsession. The RR4 and RR12 conditions allowed the greatest maintenance of velocity, while the RR4, RR12, and VL5% had a moderate, significantly greater mean heart rate than the traditional condition. Trivial, nonsignificant differences between all conditions were observed in dRPE of the legs and breathlessness and FVP profiles. These findings indicate that rest redistribution can allow for greater maintenance of sprint velocity and heart rate, without altering perceived effort during repeated sprint training. In addition, velocity-loss thresholds may be a feasible method of prescription if athletes have diverse physical qualities and reductions in sprint performance during repeated sprint training are undesirable. Practitioners should consider these outcomes when designing repeated sprint training sessions because the strategic use of these methods can alter sprint performance and internal load without changing perceptions of intensity.