Potyviruses are one of the most limiting viruses for the potato ( Solanum tuberosum and S. phureja ) production worldwide, being PVY, PVV and PVA the most prevalent species. In… Click to show full abstract
Potyviruses are one of the most limiting viruses for the potato ( Solanum tuberosum and S. phureja ) production worldwide, being PVY, PVV and PVA the most prevalent species. In this work, we tested the presence of these viruses in four commercial S. tuberosum cv. Diacol-Capiro and S. phureja cv. Criolla-Colombia plots in eastern Antioquia using RT-PCR and Sanger sequencing of the coat protein (CP) region, as well as next generation sequencing (NGS) for S. tuberosum samples. Our results revealed the presence of a PVV strain infecting S. phureja with high sequence identity (>99%) to lineage PVV Phu previously reported in Antioquia. Three strains of PVY (PVY N , PVY NTN and PVY O ) were found to infect S. tuberosum . The presence of these three PVY strains was confirmed by NGS, allowing the assembly of their complete genomes. This is the first report of the full genome sequence of PVY o strain in Colombia. These findings highlight the need to adjust the tuber-seed certification programs currently implemented in the country.
               
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