LAUSR

Plant cell suspension cultures have been proposed as alternative platforms for the expression of recombinant proteins with therapeutic application because of its potential advantages over traditional bacterial and mammalian cells-based platforms. In this work, a protocol for the establishment of potato suspensions (S. tuberosum ) genetically modified with Cry 1Ac gene was obtained and the kinetics of biomass and recombinant protein production were characterized. Internodal explants and MS medium supplemented with 2.0 mg L -1 of 2,4- D showed the best response in terms of callus formation. The maximum specific growth rate for suspensions was 0.12 d -1 , with a maximum biomass concentration of 1,41 g L -1 at the end of exponential phase. This biomass concentration was improved to 3.94 g L -1 bydoubling the concentration of NO3 - and NH4 + in the culture medium.