LAUSR

In this study, in order to investigate the diversity of fungi in the roots of Machilus pauhoi in different age, we investigated the fungi diversity and community structure in the roots of Machilus pauhoi in the age of 2-year-old, 5-year-old, 8-year-old and 20-year-old by Illumina Hiseq sequencing platform. 424 fungi operational taxonomic units (OTU) were obtained. There was no significant difference in fungi abundance and diversity index between the age of 2-year-old, 5-year-old and 20-year-old, but they were higher than those in the age of 8-year-old. On phylum level, Ascomycota, Basidiomycota and Glomeromycota were the dominant species with a relative abundance of more than 1%. Besides, with the increase of age, the fungi community in the roots of Machilus pauhoi showed a certain degree of change while then decreased to the equivalent level in the young age. On genus level, Clitocybula, Chaetosphaeria, Phylchara and Ochroconis were the dominant species, accounting for more than 10% of the investigated species. The specific species of Phyllachora appeared in the highest proportion in 8-yearold roots. Abundance heat map analysis showed that the fungi communities in the 2-year-old and 20-yearold roots were clustered into one category and those in the 5-year-old and 8-year-old were classified as another group. These results indicated that the fungi community in the roots of Machilus pauhoi was greatly affected by the growth age.