LAUSR

Potato is the most important indispensable part of human food. Cold induced sweetening is a phenomenon in which reducing sugars react with free available amino acid and give brown to black color when fried at high temperature which is not acceptable at end consumers. Moreover, acrylamide a potent carcinogenic is also produced during this process. So many wild and cultivated species have been utilized for screening the best potato germplasm against many potato diseases and quality but only a small sample of the available biodiversity has been exploited. An effort was made in this study to sort out the best potato germplasm from five different wild families on the basis of fine Molecular screening at different research station against cold induced sweetening. Crosses were made between potato families and then stored at 4 °C. Total RNA was isolated for Quantification of mRNA. Protein was isolated and invertase activity was measured through Plate reader at 5.9 . Sucrose and reducing sugars were measured through High Performance Liquid Chromatography. After 4 months of 4 °C storage incubation families were screened on the basis of chips color, invertase enzyme activity, Sucrose, reducing sugars contents and mRNA expression level. Resistance against cold induced sweetening was genotype and trial dependent especially for chip color. Invertase activity was corresponding with genotypes and trails. Locations with good chip color were having low invertase action and reducing sugars along with low mRNA expression of invertase enzyme while at other locations vice versa. Same genotype showed variable resistance at different trial satiations and conclusively invertase activity was genotypes and trials dependent. Significant trial effects were detected; all subsequent analyses were carried by trial.